Sure you can, but you have a little work to do.
This query lists users per database :
db_name() as dbname
, sp.name as login
, dp.name as [user]
from sys.database_principals dp
inner join sys.server_principals sp on sp.principal_id = dp.principal_id
WHERE dp.type_desc = ''SQL_USER''
Run it on your test and prod server and then :
- compare with Excel for eample
- import your prod users to your dev server (you will have to store the results in a table and then use Import Data wizard in Management Studio)
and compare with a FULL JOIN