I am assuming you meant Logins and not Users. Users are at the database level and are stored within the databases themselves so should come over when you restore the backups to the new instance.

To bring the Server Logins across you'll want to make sure you maintain the SIDs for the SQL Server-authentication-based Logins, else the Users in all your databases linked to those Logins on the new instance will be orphaned. You can do this by following this article:

How to transfer logins and passwords between instances of SQL Server