User permissions are stored in the database. If i take a backup of MyDatabase and restore it onto a different server all the permissions are going to be the same. the problem comes in when you create users on the new database machine, you will end up with "Orphaned" users in the database. You have two options you can use sp_help_revlogin or you can use ALTER USER http://msdn.microsoft.com/en-us/library/ms189828.aspx to map the orphaned users to a SQL Server login. Once you have mapped the Logins to Database users (or using sp_help_revlogin you do not have to go through this step as the SID's of the users will remain the same) all the permissions will be there.