hi ,

1. Check for missing indexes

2. Do you running rebuild index\reorg ,update statistics on weekend?

3.Try to find out which queries taking more cpu time from which application ?

4. Check for query optimization

5. Check the mdf files and ldf files in the same location

6. Run perfmon and check memory bottleneck and cpu bottleneck

7. Run a trace and find which queries are running during the site slowness

8 .Ask application team to check all app servers in load balancer or not ?

9.check for I\O operations [disk read and write per sec]

Thanks

Lavanya