I've got the exact same problem.
I'm on Windows XP professional and using my laptop for development.
I've got environment variables point to a Work and Source directory
The work directory is C:\Universe\Tax
The Source directory c:\TmpSource\Tax
When I run the package through BIDS, the first task is a FileSystem Task - delete directory contents on the work directory - this is successful (although there are no files)
The next task is a file system task to copy from C:\TmpSource\Tax to C:\Universe\Tax
This files with Access to the path 'C:\tmpsource\Tax' is denied
I can browse the source directory successfully through using run
Any ideas?